NixI

Description

Phage-inducible chromosomal island-like elements (PLEs) are chromosomally-integrated phage parasites described in Vibrio cholerae (N/A) . PLEs excise in response to infection by phage ICP1 and halt its progeny production. PLE halts ICP1 infection by redirecting virion packaging and interfering with ICP1 genome replication (N/A) . NixI is a PLE-encoded nuclease that nicks the ICP1 genome at specific sites preventing transition to the rolling circle replication (RCR) (N/A) .

Molecular mechanisms

The NixI endonuclease cleaves the ICP1 genome at the GNAANCTT motif (N/A) . It creates nicks and does not cause double-stranded breaks (N/A) .

Example of genomic structure

The NixI is composed of 2 proteins: NixI and Stix.

Here is an example found in the RefSeq database:

The NixI system in Apilactobacillus kunkeei (GCF_001314945.1, NZ_CP012920) is composed of 1 protein: NixI (WP_158520138.1)

Distribution of the system among prokaryotes

Structure

Experimental validation

      
graph LR;
    Legault_2022[LeGault et al., 2022] --> Origin_0
    Origin_0[Vibrio cholerae 
WP_045177897.1] --> Expressed_0[Vibrio cholerae]
    Expressed_0[Vibrio cholerae] ----> ICP1
    subgraph Title1[Reference]
        Legault_2022
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        ICP1
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none