NixI
Description
Phage-inducible chromosomal island-like elements (PLEs) are chromosomally-integrated phage parasites described in Vibrio cholerae (N/A) . PLEs excise in response to infection by phage ICP1 and halt its progeny production. PLE halts ICP1 infection by redirecting virion packaging and interfering with ICP1 genome replication (N/A) . NixI is a PLE-encoded nuclease that nicks the ICP1 genome at specific sites preventing transition to the rolling circle replication (RCR) (N/A) .
Molecular mechanisms
The NixI endonuclease cleaves the ICP1 genome at the GNAANCTT motif (N/A) . It creates nicks and does not cause double-stranded breaks (N/A) .
Example of genomic structure
The NixI is composed of 2 proteins: NixI and Stix.
Here is an example found in the RefSeq database:
The NixI system in Apilactobacillus kunkeei (GCF_001314945.1, NZ_CP012920) is composed of 1 protein: NixI (WP_158520138.1)