Abi2

Contributors: Marian Dominguez-Mirazo

Description

The Abi2 system corresponds to a group of abortive infection systems: AbiD, AbiD1 and AbiF.

The Abi2/AbiD1 system of lactococci is composed of a single protein Abi_2 () . Expression of the protein is toxic to the bacteria and is therefore highly regulated. Expression of the protein is triggered by the orf1 of phage bIL66 which results in inhibition of the phage RuvC-like endonuclease activity and stops phage multiplication.

Molecular mechanism

For AbiD1 () (AAA79209), the expression of the protein is toxic to the bacteria and is therefore highly regulated. Expression of the protein is triggered by the orf1 of phage bIL66 which results in inhibition of the phage RuvC-like endonuclease activity and stops phage multiplication.

Example of genomic structure

The Abi2 is composed of 1 protein: Abi_2.

Here is an example found in the RefSeq database:

The Abi2 system in Veillonella parvula (GCF_002005185.1, NZ_CP019721) is composed of 1 protein: Abi_2 (WP_077707938.1)

Molecular mechanisms

As far as we are aware, the molecular mechanisms remain unclear.

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the Abi2 is detected in 1231 genomes (5.4 %).

The system was detected in 202 different species.

Proportion of genome encoding the Abi2 system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

Abi2/AbiD

Example 1

Experimental validation

As Abi2 is a group of different Abortive infection systems, here is the experimental validation of AbiD:

      
graph LR;
    Chopin_2005[Chopin et al., 2005] --> Origin_0
    Origin_0[lactococcal plasmid  
AAA63619.1] --> Expressed_0[lactococci]
    Expressed_0[lactococci] ----> 936 & c2 & P335
    subgraph Title1[Reference]
        Chopin_2005
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        936
        c2
        P335
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1016/j.mib.2005.06.006
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10.1128/jb.177.13.3824-3829.1995
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