Borvo

Contributors: Héloïse Georjon

Description

Borvo is a single-gene anti-phage system that was identified through bioinformatic prediction and experimental validation () .

Molecular mechanisms

Mutations in the phage DNA polymerase can allow phages to escape Borvo defense, indicating that it could be the trigger of the system () . Borvo is a suspected abortive infection () . However, as far as we are aware, the precise molecular mechanism of Borvo is unknown.

Example of genomic structure

The Borvo is composed of 1 protein: BovA.

Here is an example found in the RefSeq database:

The Borvo system in Enterobacter cloacae complex sp. ECL78 (GCF_019056615.1, NZ_CP077660) is composed of 1 protein: BovA (WP_126517459.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the Borvo is detected in 177 genomes (0.78 %).

The system was detected in 79 different species.

Proportion of genome encoding the Borvo system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

Borvo

Example 1

Experimental validation

      
graph LR;
    Millman_2022[Millman et al., 2022] --> Origin_0
    Origin_0[Escherichia coli 
2646127138] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> T5 & SECphi4 & SECphi6 & SECphi18
    subgraph Title1[Reference]
        Millman_2022
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        T5
        SECphi4
        SECphi6
        SECphi18
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1016/j.chom.2022.09.017
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10.1016/j.cell.2023.02.029
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