CBASS

Example of genomic structure

A total of 4 subsystems have been described for the CBASS system.

Here are some examples found in the RefSeq database:

The CBASS_I system in Vibrio fluvialis (GCF_018140575.1, NZ_CP073273) is composed of 2 proteins Effector_4TM_S_4TM (WP_212571187.1) Cyclase_SMODS (WP_203534033.1)

The CBASS_II system in Klebsiella oxytoca (GCF_002072655.1, NZ_CP020358) is composed of 4 proteins Sensing_SAVED (WP_080528222.1) Jab (WP_071681958.1) AG_E1_ThiF (WP_080528223.1) Cyclase_II (WP_061351239.1)

The CBASS_III system in Conexibacter sp. DBS9H8 (GCF_023330685.1, NZ_CP097128) is composed of 5 proteins Sensing_SAVED (WP_249010498.1) Cyclase_II (WP_249010499.1) bacHORMA_2 (WP_249010500.1) HORMA (WP_249010501.1) TRIP13 (WP_249010502.1)

The CBASS_IV system in Flavobacterium alkalisoli (GCF_008000935.1, NZ_CP042831) is composed of 5 proteins OGG (WP_147582669.1) Cyclase_II (WP_147582670.1) TGT (WP_147582671.1) QueC (WP_147582672.1) Effector_2TM_Sa_NUDIX (WP_147582673.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the CBASS is detected in 2914 genomes (12.78 %).

The system was detected in 1290 different species.

Proportion of genome encoding the CBASS system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

CBASS_I

Example 1

CBASS_II

Example 1

CBASS_III

Example 1

CBASS_IV

Example 1

Experimental validation

      
graph LR;
    Cohen_2019[Cohen et al., 2019] --> Origin_0
    Origin_0[Vibrio cholerae 
WP_001901330.1, WP_001133548.1,
WP_001884104.1, WP_223225479.1] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> P1 & T2
    Cohen_2019[Cohen et al., 2019] --> Origin_1
    Origin_1[Escherichia coli] --> Expressed_1[Escherichia coli]
    Expressed_1[Escherichia coli] ----> P1 & T2 & T4 & T5 & T6 & LambdaVir
    Lowey_2020[Lowey et al., 2020] --> Origin_2
    Origin_2[Enterobacter cloacae 
WP_032676399.1, WP_032676400.1,
WP_050010101.1, WP_157903655.1] --> Expressed_2[Escherichia coli]
    Expressed_2[Escherichia coli] ----> T2 & T7
    Huiting_2022[Huiting et al., 2023] --> Origin_3
    Origin_3[Pseudomonas aeruginosa] --> Expressed_3[Pseudomonas aeruginosa]
    Expressed_3[Pseudomonas aeruginosa] ----> PaMx41 & PaMx33 & PaMx35 & PaMx43
    subgraph Title1[Reference]
        Cohen_2019
        Lowey_2020
        Huiting_2022
end
    subgraph Title2[System origin]
        Origin_0
        Origin_1
        Origin_2
        Origin_3
end
    subgraph Title3[Expression species]
        Expressed_0
        Expressed_1
        Expressed_2
        Expressed_3
end
    subgraph Title4[Protects against]
        P1
        T2
        P1
        T2
        T4
        T5
        T6
        LambdaVir
        T2
        T7
        PaMx41
        PaMx33
        PaMx35
        PaMx43
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1016/j.molcel.2019.12.009
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10.1016/j.molcel.2021.10.020
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10.1038/s41564-020-0777-y
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10.1038/s41586-019-1605-5
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10.1038/s41586-020-2719-5
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