dGTPase

Contributors: Aude Bernheim

Description

dGTPase is a family of proteins discovered in () . It degrades dGTP into phosphate-free deoxyguanosine. It was suggested that these "bacterial defensive proteins deplete deoxynucleotides_ from the_ nucleotide pool during phage infection, thus starving the phage of an essential DNA building block and halting its replication". The mechanism is remindful of the mechanism of SAMHD1 in humans.

Molecular mechanism

dGTPase degrades dGTP into phosphate-free deoxyguanosine. Phage mutants that overcome this defense carry mutations in phage-RNAP-modifying proteins suggesting, that "phage-mediated inhibition of host transcription may be involved in triggering the activation of bacterial dNTP-depletion".

Example of genomic structure

The dGTPase is composed of 1 protein: Sp_dGTPase.

Here is an example found in the RefSeq database:

The dGTPase system in Citrobacter sp. RHBSTW-00986 (GCF_013783065.1, NZ_CP056202) is composed of 1 protein: Sp_dGTPase (WP_048216953.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the dGTPase is detected in 1531 genomes (6.71 %).

The system was detected in 449 different species.

Proportion of genome encoding the dGTPase system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

dGTPase

Example 1

Experimental validation

      
graph LR;
    Tal_2022[Tal et al., 2022] --> Origin_0
    Origin_0[Escherichia coli 
2704680458] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> T5 & SECphi4 & SECphi6 & SECphi18 & SECphi27 & T7
    Tal_2022[Tal et al., 2022] --> Origin_1
    Origin_1[Mesorhizobium ssp. 
2522901616] --> Expressed_1[Escherichia coli]
    Expressed_1[Escherichia coli] ----> SECphi4 & SECphi6 & SECphi18 & SECphi27 & T7
    Tal_2022[Tal et al., 2022] --> Origin_2
    Origin_2[Pseudoalteromonas luteoviolacea 
2731093246] --> Expressed_2[Escherichia coli]
    Expressed_2[Escherichia coli] ----> T5 & SECphi4 & SECphi6 & SECphi18 & SECphi27 & T2
    Tal_2022[Tal et al., 2022] --> Origin_3
    Origin_3[Shewanella putrefaciens 
2524134135] --> Expressed_3[Escherichia coli]
    Expressed_3[Escherichia coli] ----> T5 & SECphi4 & SECphi6 & SECphi18 & SECphi27 & T2 & T6 & T7 & SECphi17
    subgraph Title1[Reference]
        Tal_2022
end
    subgraph Title2[System origin]
        Origin_0
        Origin_1
        Origin_2
        Origin_3
end
    subgraph Title3[Expression species]
        Expressed_0
        Expressed_1
        Expressed_2
        Expressed_3
end
    subgraph Title4[Protects against]
        T5
        SECphi4
        SECphi6
        SECphi18
        SECphi27
        T7
        SECphi4
        SECphi6
        SECphi18
        SECphi27
        T7
        T5
        SECphi4
        SECphi6
        SECphi18
        SECphi27
        T2
        T5
        SECphi4
        SECphi6
        SECphi18
        SECphi27
        T2
        T6
        T7
        SECphi17
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1038/s41564-022-01158-0
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