FS_HsdR_like

Contributors: Florian Tesson

Description

FS_HsdR_like or HsdR_like was discovered in 2022 () inside the PICI KpCIC51 in Klebsiella pneumoniae.

The HsdR_like system is also effective for blocking conjugation.

The HsdR_like system is composed of tree genes. The first protein encodes a DUF6731 domain and is named after it. A hypothetical protein and the HsdR protein with an N terminal HEPN domain and C terminal Zn finger domain.

Molecular mechanism

As far as we are aware, the molecular mechanism is unknown.

Example of genomic structure

The FS_HsdR_like is composed of 3 proteins: HdrR, DUF6731 and HP.

Here is an example found in the RefSeq database:

The FS_HsdR_like system in Klebsiella pneumoniae (GCF_009661415.2, NZ_CP062997) is composed of 3 proteins DUF6731 (WP_048981062.1) HP (WP_048981064.1) HdrR (WP_153928365.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the FS_HsdR_like is detected in 39 genomes (0.17 %).

The system was detected in 15 different species.

Proportion of genome encoding the FS_HsdR_like system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

FS_HsdR_like_HP-plddts_74.75219.html

Example 1

FS_HsdR_like_HdrR-plddts_88.7069.html

Example 1

Experimental validation

      
graph LR;
    Fillol-Salom_2022[Fillol-Salom et al., 2022] --> Origin_0
    Origin_0[Klebsiella pneumoniae 
QFH39804.1, QFH39805.1, QFH39806.1] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> T5 & Lambda & HK97 & HK544 & HK544 & HK578 & T7
    Fillol-Salom_2022[Fillol-Salom et al., 2022] --> Origin_0
    Origin_0[Klebsiella pneumoniae 
QFH39805.1, RXS01985.1] --> Expressed_1[Salmonella enterica]
    Expressed_1[Salmonella enterica] ----> P22 & BTP1 & ES18
    subgraph Title1[Reference]
        Fillol-Salom_2022
end
    subgraph Title2[System origin]
        Origin_0
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
        Expressed_1
end
    subgraph Title4[Protects against]
        T5
        Lambda
        HK97
        HK544
        HK544
        HK578
        T7
        P22
        BTP1
        ES18
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1016/j.cell.2022.07.014
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