MMB gp29-gp30

Contributors: Hugo Vaysset

Description

MMB gp29-gp30 is a defense system that was found in the MichelleMyBell (MMB) temperate mycobacteriophage. It is an example of prophage-mediated defense () .

Molecular mechanisms

To the extent of our knowledge, the precise mechanism of action of MMB gp29-gp30 is not known. The system was shown to protect against lytic infection by temperate phage (e.g. phage Tweety) and does not act through abortive infection. Cells that survive infection tend to become lysogens for the infecting phage. It was also shown that the expression of gp29 alone was toxic in M. smegmatis () .

Example of genomic structure

The MMB_gp29_gp30 is composed of 2 proteins: gp29 and gp30.

Here is an example found in the RefSeq database:

The MMB_gp29_gp30 system in Mycolicibacterium insubricum (GCF_010731615.1, NZ_AP022618) is composed of 2 proteins gp29 (WP_234805905.1) gp30 (WP_083031848.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the MMB_gp29_gp30 is detected in 5 genomes (0.02 %).

The system was detected in 4 different species.

Proportion of genome encoding the MMB_gp29_gp30 system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

MMB_gp29_gp30

Example 1

Experimental validation

      
graph LR;
    Dedrick_2017[Dedrick et al., 2017] --> Origin_0
    Origin_0[Mycobacterium MMB phage 
YP_009006630.1, YP_009006631.2] --> Expressed_0[Mycobacterium smegmatis mc2 155]
    Expressed_0[Mycobacterium smegmatis mc2 155] ----> Bxz2 & Tweety & TM4
    subgraph Title1[Reference]
        Dedrick_2017
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        Bxz2
        Tweety
        TM4
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1038/nmicrobiol.2016.251
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