PD-T4-3

Contributors: Ernest Mordret

Description

PD-T4-3 is a single gene system isolated during an experimental screen of fosmids derived from 71 strains of diverse E.coli, and cloned back in a derivative of K12. Fragments containing the PD-T4-3 gene were shown to provide resistance to phage T4. The protein is characterized by a GIY-YIG nuclease domain, and mutations inactivating this domain abolished the resistance phenotype. It was originally found on a µ-like prophage

Molecular mechanism

the molecular mechanism of this system is unknown

Example of genomic structure

The PD-T4-3 is composed of 1 protein: PD-T4-3.

Here is an example found in the RefSeq database:

The PD-T4-3 system in Morganella morganii (GCF_018802485.1, NZ_CP064826) is composed of 1 protein: PD-T4-3 (WP_214179544.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the PD-T4-3 is detected in 1314 genomes (5.76 %).

The system was detected in 172 different species.

Proportion of genome encoding the PD-T4-3 system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

PD-T4-3

Example 1

Experimental validation

      
graph LR;
    Vassallo_2022[Vassallo et al., 2022] --> Origin_0
    Origin_0[Escherichia coli 
RCO27183.1] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> T2 & T4 & T6
    subgraph Title1[Reference]
        Vassallo_2022
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        T2
        T4
        T6
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    
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