PD-T4-6

Contributors: Marian Dominguez-Mirazo

Description

The PD-T4-6 system is composed of a single protein. It was discovered via a selection screening of 71 E. coli strains challenged with diverse phage. The name stands from Phage Defense (PD) and the phage with which the strain was challenged (T4) () . The system has been identified as an Abortive Infection (Abi) system (, ) . The protein was found within a P2-like prophage and contains a predicted Der/Thr kinase domain. Site-specific mutation in the domain reduces phage protection () .

Molecular mechanisms

As far as we are aware, the molecular mechanism is unknown.

Example of genomic structure

The PD-T4-6 is composed of 1 protein: PD-T4-6.

Here is an example found in the RefSeq database:

The PD-T4-6 system in Streptococcus ruminantium (GCF_021654555.1, NZ_AP025333) is composed of 1 protein: PD-T4-6 (WP_024532776.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the PD-T4-6 is detected in 88 genomes (0.39 %).

The system was detected in 18 different species.

Proportion of genome encoding the PD-T4-6 system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

PD-T4-6

Example 1

Experimental validation

      
graph LR;
    Vassallo_2022[Vassallo et al., 2022] --> Origin_0
    Origin_0[Escherichia coli 
RRM76169.1] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> T2 & T4 & T6
    subgraph Title1[Reference]
        Vassallo_2022
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        T2
        T4
        T6
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1038/s41564-022-01219-4
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10.1016/j.mib.2023.102312
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