PD-T7-5

Contributors: Hugo Vaysset

Description

PD-T7-5 is a defense system composed of a single protein which was discovered in () . Its antiphage activity was assessed by heterologous expression in E. coli against T7 and to reduce the size of lysis plaques of T3 () . PD-T7-5 contains a PD(D/E)XK nuclease domain like PD-T7-1.

Molecular mechanism

As far as we are aware, the molecular mechanism is unknown. However, the presence of a PD(D/E)XK domain in PD-T7-5 suggests a mechanism of action via DNA degradation () .

Example of genomic structure

The PD-T7-5 system is composed of one protein: PD-T7-5.

Here is an example found in the RefSeq database:

The PD-T7-5 system in Shewanella putrefaciens (GCF_019599085.1, NZ_CP080633) is composed of 1 protein: PD-T7-5 (WP_220590848.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the PD-T7-5 is detected in 336 genomes (1.47 %).

The system was detected in 129 different species.

Proportion of genome encoding the PD-T7-5 system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

PD-T7-5

Example 1

Experimental validation

      
graph LR;
    Vassallo_2022[Vassallo et al., 2022] --> Origin_0
    Origin_0[Escherichia coli 
RRM82777.1] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> SECphi17 & T3 & T7
    subgraph Title1[Reference]
        Vassallo_2022
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        SECphi17
        T3
        T7
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1038/s41564-022-01219-4
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