RloC

Contributors: Aude Bernheim, Florian Tesson

Description

RloC is a distant homolog of the tRNALys anticodon nuclease (ACNase) PrrC. RloC shares the ABC ATPase motifs and catalytic ACNase triad with PrrC, but it possesses a unique zinc-hook/coiled-coil insert. This distinctive feature results in its ATPase domain bearing similarity to Rad50 and other DNA repair proteins.Contrary to PrrC RloC is rarely linked to a type I R-M system. RloC activity was not directly demonstrated on phages but hypothesized through its similar features to PrrC () .

This system was not experimentally validated but is still considered as a defense system because of its homology with PrrC and its abundance in defense islands. Of the RloC system detected by DefenseFinder around 40% are close to at least one known defense system (20 proteins on each side).

Molecular Mechanism

RloC was shown to function by tRNA cleavage (wobble nucleotide-excising and Zn++-responsive tRNase) () . However, such activity was not directly shown on a phage Zinc hook seems to regulate the nuclease function.

Example of genomic structure

The RloC is composed of 1 protein: RloC.

Here is an example found in the RefSeq database:

The RloC system in Pseudomonas aeruginosa (GCF_000981825.1, NZ_CP011317) is composed of 1 protein: RloC (WP_010794466.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the RloC is detected in 1944 genomes (8.53 %).

The system was detected in 919 different species.

Proportion of genome encoding the RloC system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

RloC

Example 1

Experimental validation

To our knowledge, there is no experimental validation for the RloC system.

References

10.1111/j.1365-2958.2008.06387.x
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10.1006/jmbi.1995.0343
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