Rst_gop_beta_cll

Contributors: Florian Tesson

Description

The gop-beta-cll system was shown to have an antiphage activity in 2022 () against Escherichia coli phage lambda and P1.

The gop-beta-cll was first described as a nonessential region of the bacteriophage P4 () .

This system is composed of 3 genes: gop, beta and cll.

Molecular mechanism

As far as we are aware, the molecular mechanism is unknown.

The production of the gop protein without beta is toxic () suggesting a toxin antitoxin mechanism () .

Example of genomic structure

The Rst_gop_beta_cll is composed of 3 proteins: beta, cll and gop.

Here is an example found in the RefSeq database:

The Rst_gop_beta_cll system in Klebsiella grimontii (GCF_019334465.1, NZ_CP079754) is composed of 3 proteins gop (WP_189095150.1) beta (WP_189095151.1) cll (WP_219192256.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the Rst_gop_beta_cll is detected in 37 genomes (0.16 %).

The system was detected in 15 different species.

Proportion of genome encoding the Rst_gop_beta_cll system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

Rst_gop_beta_cll

Example 1

Experimental validation

      
graph LR;
    Rousset_2022[Rousset et al., 2022] --> Origin_0
    Origin_0[Enterobacteria phage P4 
WP_105493229.1, ENG39111.1,
NP_042032.1] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> Lambda & P1
    subgraph Title1[Reference]
        Rousset_2022
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        Lambda
        P1
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1016/j.chom.2022.02.018
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10.1128/jvi.64.1.24-36.1990
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