Rst_HelicaseDUF2290

Contributors: Ernest Mordret

Description

The Rst_HelicaseDUF2290 system was discovered during a screen for defense systems targeted at hotspots of antiphage activity in phages and phage-satellites. It is composed of 2 proteins, a helicase and a protein with the domain DUF2290.

Molecular mechanism

As far as we are aware, the molecular mechanism is unknown.

Example of genomic structure

The Rst_HelicaseDUF2290 is composed of 2 proteins: Helicase and DUF2290.

Here is an example found in the RefSeq database:

The Rst_HelicaseDUF2290 system in Klebsiella quasipneumoniae (GCF_003020825.1, NZ_CP023478) is composed of 2 proteins Helicase (WP_046623504.1) DUF2290_Pers (WP_046623503.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the Rst_HelicaseDUF2290 is detected in 207 genomes (0.91 %).

The system was detected in 123 different species.

Proportion of genome encoding the Rst_HelicaseDUF2290 system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

Rst_HelicaseDUF2290

Example 1

Experimental validation

      
graph LR;
    Rousset_2022[Rousset et al., 2022] --> Origin_0
    Origin_0[Klebsiella pneumoniae P4 loci 
WP_046623503.1, WP_046623504.1] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> T7
    subgraph Title1[Reference]
        Rousset_2022
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        T7
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1016/j.chom.2022.02.018
no authors
no containerTitle ()