SanaTA

Contributors: Alba Herrero del Valle

Description

The SanaTA system is composed of 2 proteins: SanaT and, SanaA, where SanaT encodes a toxin and SanaA encodes an antitoxin to form a toxin-antitoxin (TA) system. The toxin protein, SanaT, contains a nucleotidyltransferase domain (PF08843). This system provides resistance against mutated T7 phages that lack the nonessential 4.5 gene in Shewanella sp. ANA-3. Sberro et al. showed that the defensiveness of sanaTA depends on the cleavage of SanaA by the Lon system, a protease that degrades the antitoxin to allow the activity of the toxin () .

Molecular mechanism

It has been shown to function as a toxin-antitoxin (TA) system.

Example of genomic structure

The SanaTA is composed of 2 proteins: SanaA and SanaT.

Here is an example found in the RefSeq database:

The SanaTA system in Euzebyella marina (GCF_003687485.1, NZ_CP032050) is composed of 2 proteins SanaA (WP_121848345.1) SanaT (WP_121848346.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the SanaTA is detected in 992 genomes (4.35 %).

The system was detected in 519 different species.

Proportion of genome encoding the SanaTA system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

SanaTA

Example 1

Experimental validation

      
graph LR;
    Sberro_2013[Sberro et al., 2013] --> Origin_0
    Origin_0[Shewanella sp. ANA-3 
639720518, 639720519] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> T7
    subgraph Title1[Reference]
        Sberro_2013
end
    subgraph Title2[System origin]
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
end
    subgraph Title4[Protects against]
        T7
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1016/j.molcel.2013.02.002
no authors
no containerTitle ()