ShosTA

Contributors: Florian Tesson

Description

ShosTA system was first described as a Toxin/Antitoxin system in 2012 () without demonstration of antiphase activity. In 2022, a paper described the same system as "DprA + PRTase" inside P2-like prophages and proved its antiphage activity. Finally, the antiphage activity was also proved in another study with the original name ShosTA () .

This system is composed of two proteins: ShosT and ShosA encoding for Hydrolase/PRTase and DprA (nucleotide binding) respectively.

Molecular mechanism

The ShosTA system is a toxin (ShosT) antitoxin (ShosA) system. The domains of ShosT (Hydrolase and PRTase) allow us to hypothesize toxicity linked to host protein degradation.

Example of genomic structure

The ShosTA is composed of 2 proteins: ShosA and ShosT.

Here is an example found in the RefSeq database:

The ShosTA system in Enterobacter cloacae (GCF_009707405.1, NZ_CP046116) is composed of 2 proteins ShosT (WP_129253192.1) ShosA (WP_129253194.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the ShosTA is detected in 535 genomes (2.35 %).

The system was detected in 240 different species.

Proportion of genome encoding the ShosTA system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

ShosTA

Example 1

Experimental validation

      
graph LR;
    Millman_2022[Millman et al., 2022] --> Origin_0
    Origin_0[Escherichia coli 
2564403099, 2564403100] --> Expressed_0[Escherichia coli]
    Expressed_0[Escherichia coli] ----> LambdaVir & SECphi4 & SECphi6 & SECphi18 & T7
    Rousset_2022[Rousset et al., 2022] --> Origin_1
    Origin_1[Escherichia coli P2 loci] --> Expressed_1[Escherichia coli]
    Expressed_1[Escherichia coli] ----> Lambda & T7
    subgraph Title1[Reference]
        Millman_2022
        Rousset_2022
end
    subgraph Title2[System origin]
        Origin_0
        Origin_1
end
    subgraph Title3[Expression species]
        Expressed_0
        Expressed_1
end
    subgraph Title4[Protects against]
        LambdaVir
        SECphi4
        SECphi6
        SECphi18
        T7
        Lambda
        T7
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1016/j.chom.2022.09.017
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10.1016/j.chom.2022.02.018
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10.1101/gr.133850.111
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