Stk2

Contributors: Héloïse Georjon, Hugo Vaysset, Florian Tesson

Description

Eukaryotic-like serine/threonine kinases have a variety of functions in prokaryotes. Recently, a single-gene system (Stk2) encoding for a Serine/threonine kinase from Staphylococcus epidermidis has been found to have anti-phage activity both in its native host and in a heterologous S.aureus host () .

Molecular mechanism

Stk2 is an Abortive infection system, which triggers cell death upon phage infection, probably through phosphorylation of diverse essential cellular pathways () . Stk2 was shown to detect a phage protein named Pack, which was proposed to be involved in phage genome packaging () .

Example of genomic structure

The Stk2 is composed of 1 protein: Stk2.

Here is an example found in the RefSeq database:

The Stk2 system in Bacillus rugosus (GCF_023238245.1, NZ_CP096590) is composed of 1 protein: Stk2 (WP_248602500.1)

Distribution of the system among prokaryotes

Among the 22,803 complete genomes of RefSeq, the Stk2 is detected in 140 genomes (0.61 %).

The system was detected in 29 different species.

Proportion of genome encoding the Stk2 system for the 14 phyla with more than 50 genomes in the RefSeq database.

Structure

Stk2

Example 1

Experimental validation

      
graph LR;
    Depardieu_2016[Depardieu et al., 2016] --> Origin_0
    Origin_0[Staphylococcus epidermidis 
WP_001001347.1] --> Expressed_0[Staphylococcus epidermidis]
    Expressed_0[Staphylococcus epidermidis] ----> CNPx
    Depardieu_2016[Depardieu et al., 2016] --> Origin_0
    Origin_0[Staphylococcus epidermidis 
WP_001001347.1] --> Expressed_1[Staphylococcus aureus]
    Expressed_1[Staphylococcus aureus] ----> phage80alpha & phage85 & phiNM1 & phiNM2 & phiNM4
    subgraph Title1[Reference]
        Depardieu_2016
end
    subgraph Title2[System origin]
        Origin_0
        Origin_0
end
    subgraph Title3[Expression species]
        Expressed_0
        Expressed_1
end
    subgraph Title4[Protects against]
        CNPx
        phage80alpha
        phage85
        phiNM1
        phiNM2
        phiNM4
end
    style Title1 fill:none,stroke:none,stroke-width:none
    style Title2 fill:none,stroke:none,stroke-width:none
    style Title3 fill:none,stroke:none,stroke-width:none
    style Title4 fill:none,stroke:none,stroke-width:none

    

References

10.1016/j.chom.2016.08.010
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